qpcr retrovirus titration kit
Receive fixed retroviral transductions
A critical good thing about retroviral gene-delivery strategies is the ability to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell kind and ultimately allow you to administration the expression stage between experiments.
The way in which it really works
The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.
RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embody residual plasmid DNA, might be exactly quantified using this gear.
Virus Titration Kits
Our Titration Kits current a fast and straightforward method for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and determine viral RNA/DNA titer using qRT-PCR.
Advantages of abm’s titration kits:
- No RNA purification — saves time and eliminates inaccuracy.
- Ready-to-use reagent mix — reduces variability.
- qRT-PCR in a single step — additional delicate and proper than totally different methods.
- No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.
We moreover provide On the spot Lentivirus Titration Enjoying playing cards, which enable quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay method, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.
he Retro-X qRT-PCR Titration Gear offers a fast and straightforward method for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.
Description
abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be achieved beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better complete effectivity compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally appropriate with most qPCR gadgets.
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Quick willpower of retroviral titer
Whereas typical titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the an identical day.
Retrovirus 10x Titer-Up Kit Test Sample (0.1 mL) |
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| P909S | 101Bio | - | Ask for price |
Retro-SV40LT Retrovirus |
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| RVP010 | ABM | 1.0 ml | EUR 296.4 |
Retrovirus Mini Purification Kit |
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| K1307-10 | Biovision | each | EUR 652.8 |
Retrovirus Mini Purification Kit |
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| K1307-20 | Biovision | each | EUR 1096.8 |
Retrovirus Maxi Purification Kit |
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| K1308-10 | Biovision | each | EUR 1554 |
Retrovirus Maxi Purification Kit |
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| K1308-2 | Biovision | each | EUR 639.6 |
Retrovirus Maxi Purification Kit |
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| K1308-4 | Biovision | each | EUR 1032 |
Retrovirus Booster 50µl |
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| ABP-RVC-RBST05 | Allele Biotech | 50µl | Ask for price |
Retrovirus Booster 200µl |
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| ABP-RVC-RBST20 | Allele Biotech | 200µl | Ask for price |
Retrovirus Booster 400µl |
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| ABP-RVC-RBST40 | Allele Biotech | 400µl | Ask for price |
Retrovirus Stabilizer 50µl |
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| ABP-RVC-RSTB05 | Allele Biotech | 50µl | Ask for price |
Retrovirus Stabilizer 200µl |
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| ABP-RVC-RSTB20 | Allele Biotech | 200µl | Ask for price |
Retrovirus Stabilizer 400µl |
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| ABP-RVC-RSTB40 | Allele Biotech | 400µl | Ask for price |
iPS Cell Line (Retrovirus) |
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| ASE-9101 | Applied StemCell | 1 vial of ^2.5X10^5 cells | EUR 516.84 |
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Description: 6 month |
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QuickTiter Retrovirus Quantitation Kit |
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| VPK-120 | Cell Biolabs | 20 assays | EUR 853.2 |
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Description: Our QuickTiter Retrovirus Quantitation Kit provides a quick method for determining retroviral titer. This assay measures the viral nucleic acid content of your retrovirus, and may be performed either before or after purification of your virus. |
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M13 Phage Titration ELISA Kit |
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| 55R-PRPHAGE | Fitzgerald | 96 tests | EUR 831.6 |
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Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles |
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Lentivirus and Retrovirus Purification Kit |
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| K1458-100 | Biovision | 100 Preps | EUR 1244.4 |
Lentivirus and Retrovirus Purification Kit |
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| K1458-20 | Biovision | 20 Preps | EUR 727.2 |
Retrovirus-related Gag Antibody |
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| 48261-100ul | SAB | 100ul | EUR 399.6 |
Retrovirus-related Gag Antibody |
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| 48261-50ul | SAB | 50ul | EUR 286.8 |
Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80155-G | Alpha Diagnostics | 1 kit | EUR 781.2 |
Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80155-M | Alpha Diagnostics | 1 kit | EUR 781.2 |
Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80150-M | Alpha Diagnostics | 1 kit | EUR 781.2 |
Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests) |
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| 80150-G3 | Alpha Diagnostics | 1 kit | EUR 781.2 |
Retrovirus Concentration Solution (100 mL) |
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| P905C | 101Bio | - | Ask for price |
Endogenous retrovirus (replication competent) PCR kit |
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| PCR-V725-48D | Bioingentech | 50T | EUR 510.96 |
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Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent) |
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Endogenous retrovirus (replication competent) PCR kit |
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| PCR-V725-96D | Bioingentech | 100T | EUR 625.8 |
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Description: An conventional PCR kit for detection of Endogenous retrovirus (replication competent) |
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Endogenous retrovirus (replication competent) PCR kit |
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| PCR-H725-48D | Bioingentech | 48T | EUR 543.6 |
Endogenous retrovirus (replication competent) PCR kit |
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| PCR-H725-96D | Bioingentech | 96T | EUR 686.4 |
pCHAC-hKLF4 Retrovirus Vector Validated |
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| ABP-SC-VCREKLF | Allele Biotech | 10µg | Ask for price |
