Abm Qpcr Retrovirus

qpcr retrovirus titration kit

Receive fixed retroviral transductions

A critical good thing about retroviral gene-delivery strategies is the ability to manage the extent of gene expression by adjusting the multiplicity of an an infection (MOI) to dictate the number of viral genomes that enter your objective cells. These titration kits allow you to make a quick correlation between the retroviral copy amount and retroviral infectivity in your cell kind and ultimately allow you to administration the expression stage between experiments.

The way in which it really works

The qRT-PCR course of entails subjecting serial dilutions of your sample and a administration RNA of acknowledged copy amount (provided) to one-step qRT-PCR. The retroviral copy amount contained in your sample can then be determined by evaluating its Ct value to the standard curve.

RNA purification and DNase I remedy steps are essential to the protocol so even viruses packaged using transient transfections, which always embody residual plasmid DNA, might be exactly quantified using this gear.

Virus Titration Kits

Our Titration Kits current a fast and straightforward method for titrating your virus. The kits make use of each a quick RNA or DNA extraction step, and determine viral RNA/DNA titer using qRT-PCR.

Advantages of abm’s titration kits:

  • No RNA purification — saves time and eliminates inaccuracy.
  • Ready-to-use reagent mix — reduces variability.
  • qRT-PCR in a single step — additional delicate and proper than totally different methods.
  • No NTC amplification — Distinctive primer design completely eliminates non-specific amplification in NTCs.

We moreover provide On the spot Lentivirus Titration Enjoying playing cards, which enable quick detection of lentiviral capsule proteins as a marker to substantiate the presence of lentivirus. Utilizing the lateral flow into immunoassay method, viral titer yields are assessed instantly inside 10 minutes using our three step protocol.

he Retro-X qRT-PCR Titration Gear offers a fast and straightforward method for measuring retrovirus titer. The kits use a quick RNA purification step sooner than quantifying the number of retroviral genome copies using qRT-PCR and TB Inexperienced utilized sciences.

Description

abm’s qPCR Retrovirus Titer Gear is a one-step assay by qPCR which does not require additional lysis or reverse transcription (RT) steps, and might be achieved beneath one hour. Designed to ship extreme sensitivity and specificity, the gear ensures minimal non-specific background and better complete effectivity compared with comparable kits within the market. ROX reference dye is obtainable separate from the MasterMix, making this gear universally appropriate with most qPCR gadgets.

 

qPCR and One-Step RT MasterMixes
qPCR and One-Step RT MasterMixes
PRODUCT COMPONENT QUANTITY
BlasTaq 2X qPCR Titer MasterMix 1.25 ml
Primer Mix 100 rxn (200 µl)
Commonplace Administration DNA 50 µl
ROX Reference Dye 15 µl
Nuclease-Free H2O 2 x 1.zero ml

 

SKU G949
Unit quantity 100 rxn
Storage State of affairs Retailer at -20°C

 

Quick willpower of retroviral titer

Whereas typical titration methods require as a lot as 10 days to complete, these titration kits require solely 4 hours and work with all MMLV-based retroviral vectors (not MSCV). Using qRT-PCR dramatically shortens the time interval between viral harvest and target-cell an an infection, allowing you to hold out every on the an identical day.

 

M13 Phage Titration ELISA Kit

55R-PRPHAGE 96 tests
EUR 831.6
Description: Enzyme Immunoassay for the Quantitative Determination of purified M13 Bacteriophage Particles

AAV5 Titration ELISA Kit (Fast)

AAV-A005H 96tests
EUR 706.2
Description: AAV5 Titration ELISA Kit(Fast) is based on ELISA sandwich method and designed to detect and quantitative determine of AAV5 capsid titration in AAV gene therapy product preparation processing. It contains AAV5 empty capsids and a pair of antibodies against AAV5. Results are obtained by four parameter logistic curve that were parallel to the standard curves obtained. The verification results indicate that this kit can be used for the quantitative determination of AAV5 capsid titration.

Rat Serum IgG detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-G 1 kit
EUR 781.2

Rat Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-M 1 kit
EUR 781.2

Mouse Serum IgM detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-M 1 kit
EUR 781.2

Mouse Serum IgG3 detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G3 1 kit
EUR 781.2

Lentivirus Titration XpressCard

ATG-LT-01-10 10 tests
EUR 195

Lentivirus Titration XpressCard

ATG-LT-01-20 20 tests
EUR 335

Lentivirus Titration XpressCard

ATG-LT-01-50 50 tests
EUR 715

Lentivirus Titration XpressCard

ATG-LT-01-6 6 tests
EUR 136

Mouse Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-A 1 kit
EUR 781.2

Mouse Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-E 1 kit
EUR 781.2

Rat Primary Antibody (IgA) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-A 1 kit
EUR 781.2

Rat Primary Antibody (IgE) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155-E 1 kit
EUR 781.2

Instant Lentivirus Titration Card

G614 10 Tests
EUR 235

Instant Lentivirus Titration Card

G615 20 Tests
EUR 405

Mouse Primary Antibody (IgG1) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G1 1 kit
EUR 781.2

Mouse Primary Antibody (IgG2a) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G2a 1 kit
EUR 781.2

Mouse Primary Antibody (IgG2b) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G2b 1 kit
EUR 781.2

Mouse Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150 1 Kit
EUR 781.2

Rat Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80155 1 Kit
EUR 781.2

Rabbit Primary Antibody (A+G+M) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80160 1 Kit
EUR 781.2

Mouse Primary Antibody (IgG1-3) detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80150-G 1 kit
EUR 781.2

baculoQUANT All-in-one virus extraction & titration kit

100602 100 reactions
EUR 580.32

Titration vessel for HI-904 (glass)

TIT1052 EACH
EUR 554.4

Ferret Primary Antibody (IgA) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-A 1 kit
EUR 781.2

Ferret Primary Antibody (IgG) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-G 1 kit
EUR 781.2

Ferret Primary Antibody (IgM) Antibody detection and titration and titration ELISA kit, Qualitative (sufficient for 500-1000 tests)

80188-M 1 kit
EUR 781.2

Automatic Potentiometric Titration System

TIT1030 EACH
EUR 6540

Titration vessel for HI-904 (assembly)

TIT1050 EACH
EUR 1003.2

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