
Peanut (Arachis hypogaea) agglutinin (PNA)
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Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply. It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins.
Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).
Biochem/Physiol Actions
Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Software program
Frequent Western Blot Protocol:
- Glycoprotein sample dimension: 500ng
- Lectin Focus: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS net web page gel
- Change gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Unit Definition
Bodily kind
Preparation Discover
Arachis hypogaea Conglutin-7 |
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1-CSB-EP757666ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
Asprosin Polyclonal Antibody (Human), HRP |
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4-PAA332Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Asprosin. This antibody is labeled with HRP. |
Vinculin (VCL) Polyclonal Antibody (Human), HRP |
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4-CAB839Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Vinculin (VCL). This antibody is labeled with HRP. |
Titin (TTN) Polyclonal Antibody (Human), HRP |
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4-PAB667Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Titin (TTN). This antibody is labeled with HRP. |
Melanotransferrin (MFI2) Polyclonal Antibody (Human), HRP |
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4-PAB677Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Melanotransferrin (MFI2). This antibody is labeled with HRP. |
Cholesterol (CH) Polyclonal Antibody (General), HRP |
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4-PAB701Ge01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Cholesterol (CH). This antibody is labeled with HRP. |
Glycocalicin (GC) Polyclonal Antibody (Human), HRP |
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4-PAB710Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Glycocalicin (GC). This antibody is labeled with HRP. |
Fascin (FSCN) Polyclonal Antibody (Human), HRP |
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4-PAB757Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Fascin (FSCN). This antibody is labeled with HRP. |
Fascin (FSCN) Polyclonal Antibody (Mouse), HRP |
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4-PAB757Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Fascin (FSCN). This antibody is labeled with HRP. |
Neprilysin (NEP) Polyclonal Antibody (Mouse), HRP |
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4-PAB785Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Neprilysin (NEP). This antibody is labeled with HRP. |
Neprilysin (NEP) Polyclonal Antibody (Rat), HRP |
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4-PAB785Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with HRP. |
Neprilysin (NEP) Polyclonal Antibody (Rat), HRP |
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4-PAB785Ra02-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Neprilysin (NEP). This antibody is labeled with HRP. |
Arginine (Arg) Polyclonal Antibody (General), HRP |
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4-PAB938Ge01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against General Arginine (Arg). This antibody is labeled with HRP. |
Epiregulin (EREG) Polyclonal Antibody (Rat), HRP |
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4-PAB945Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Epiregulin (EREG). This antibody is labeled with HRP. |
Acrosin (ACR) Polyclonal Antibody (Rat), HRP |
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4-PAB947Ra01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Rat Acrosin (ACR). This antibody is labeled with HRP. |
Hepcidin (Hepc) Polyclonal Antibody (Bovine), HRP |
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4-PAB979Bo01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Bovine Hepcidin (Hepc). This antibody is labeled with HRP. |
Hepcidin (Hepc) Polyclonal Antibody (Human), HRP |
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4-PAB979Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Hepcidin (Hepc). This antibody is labeled with HRP. |
Hepcidin (Hepc) Polyclonal Antibody (Mouse), HRP |
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4-PAB979Mu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Mouse Hepcidin (Hepc). This antibody is labeled with HRP. |