
Peanut (Arachis hypogaea) agglutinin (PNA)
Pedro
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Arachis hypogaea (Peanut) Lectin (PNA) – HRP
Affinity-purified PNA is conjugated to horseradish peroxidase (HRP) enzyme. HRP is usually used for blotting, immunoassays and immunohistochemistry methods. It is a 40 kDa protein that catalyzes the oxidation of substrates by hydrogen peroxide, resulting in a colored or fluorescent product or launch of sunshine as a byproduct of the response. This product is obtainable in a stabilized liquid kind.For substrate use of HRP-labelled protein detection we offer our Intensive ChemiLuminescence (ICLTM) tools (SKU: 20810000). ICL is a two-component substrate that accommodates a gentle luminol reply with an enhancer and a gentle peroxide buffer reply. It has a robust light emission and a sensitivity at picogram diploma that is extraordinarily acceptable for the speedy detection of HRP-conjugated proteins.
Advantages of chemiluminescent substrates over totally different detection methods incorporates a lot of exposures, blots is also reprobed, detects and quantitates a wide range of protein concentrations, and yields largest sensitivity of any on the market method.We moreover provide 3, 3, 5, 5-Tetramethyl benzidine (TMB, SKU: 42000012) and TMB OneTM (TMB 1) HRP substrate for HRP labelled detection. TMB is an acceptable substrate for the immunoassay of horseradish peroxidase and is a noncarcinogenic by-product of benzidine with an absorbance at 450 nm. Oxidation of TMB by HRP yields a colored product. s TMB 1TM is a one ingredient peroxidase substrate (SKU: 21530073).
Biochem/Physiol Actions
Abstract
Peanut (Arachis hypogaea) agglutinin (PNA) is extensively used as tumour arker it strongly recognises essentially the most cancers specific T antigen (Galbeta1–>3GalNAc-), nevertheless not its sialylated mannequin. Nonetheless, an additional specificity within the course of Galbeta1–>4GlcNAc (LacNAc), which is not tumour specific, had been attributed to PNA.
For correct interpretation of lectin histochemical outcomes we examined PNA sugar specificity using naturally occurring or semi-synthetic glycoproteins, matrix-immobilised galactosides and lectin-binding tissue glycoproteins, moderately than mono- or disaccharides as ligands. Dot-blots, swap blots or polystyrene plate coatings of the soluble glycoconjugates have been probed with horse-radish peroxidase (HRP) conjugates of PNA and totally different lectins of acknowledged specificity. Modifications of PNA-binding glycoproteins, along with selective eradicating of O-linked oligosaccharides and remedy with glycosidases revealed that Galbeta1–>4GlcNAc (LacNAc) was ineffective whereas terminal alpha-linked galactose (TAG) along with uncovered T antigen (Galbeta1–>Three GalNAc-) was fantastic as sugar moiety in glycoproteins for his or her recognition by PNA. When immobilised, melibiose was superior to lactose in PNA binding. Outcomes have been confirmed using TAG-specific human serum anti-alpha-galactoside antibody.

Software program
Frequent Western Blot Protocol:
- Glycoprotein sample dimension: 500ng
- Lectin Focus: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS net web page gel
- Change gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Unit Definition
Bodily kind
Preparation Discover
Arachis hypogaea Conglutin-7 |
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1-CSB-EP757666ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Conglutin-7 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-EP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3 expressed in E.coli |
Arachis hypogaea Arachin Ahy-3 |
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1-CSB-YP737391ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Arachin Ahy-3,partial expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-YP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in Yeast |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE(A4) | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17 expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P17 |
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1-CSB-EP331779ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P17,partial expressed in E.coli |
Arachis hypogaea Allergen Ara h 1, clone P41B |
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1-CSB-CF337356ANE | Cusabio |
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Description: Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B expressed in in vitro E.coli expression system |
Biotin [HRP] |
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DAG3197-HRP | Creative Diagnostics | 1mg | EUR 429 |
Vitamine B9 [HRP] |
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DAGDT1440-HRP | Creative Diagnostics | 1mg | EUR 1105 |
Vitamine B12 [HRP] |
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DAGT5413-HRP | Creative Diagnostics | 1mg | EUR 975 |
GAPDH Antibody - HRP |
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T0004-HRP | Affbiotech | 1ml | EUR 920 |
Description: GAPDH Mouse Monoclonal antibody detects endogenous levels of total GAPDH protein. |
CF488A Arachis hypogaes Lectin PNA (Peanut) |
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29060 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
CF568 Arachis hypogaes Lectin PNA (Peanut) |
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29061 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
CF594 Arachis hypogaes Lectin PNA (Peanut) |
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29062 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
CF640R Arachis hypogaes Lectin PNA (Peanut) |
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29063 | Biotium | 1mg | EUR 195 |
Description: Minimum order quantity: 1 unit of 1mg |
beta-Actin Antibody - HRP |
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T0022-HRP | Affbiotech | 1ml | EUR 920 |
Description: beta-Actin Mouse Monoclonal antibody detects endogenous levels of total beta-Actin protein. |
HRP-IgG Conjugation Kit |
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55R-HRP | Fitzgerald | 1 mg | EUR 705 |
Description: HRP conjugation kit for all types of IgG |
Anti-Per.C6Ð’ Affinity Purified HRP, Goat |
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PC118AF-HRP | Cygnus Technologies | 1 mg | EUR 4069 |
Description: Anti-Per.C6Ð’ Affinity Purified HRP, Goat by Cygnus Technologies is available in Europe via Gentaur. |
Rabbit Anti-Bird IgM-HRP Conjugate |
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90521-HRP | Alpha Diagnostics | 100 ul | EUR 347 |
Concanavalin A Lectin (ConA)-HRP conjugate |
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CONA15-HRP | Alpha Diagnostics | 0.5 ml | EUR 286 |
Rabbit Anti-Salmonella IgG-HRP conjugate |
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SLM11-HRP | Alpha Diagnostics | 0.5 ml | EUR 347 |
Sambucus nigra Lectin (SNA)-HRP conjugate |
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SNA15-HRP | Alpha Diagnostics | 0.5 ml | EUR 286 |
Lens culinaris Lectin (LCA)-HRP conjugate |
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LCA15-HRP | Alpha Diagnostics | 0.5 ml | EUR 286 |
Monoclonal Anti-Poly His-HRP conjugate |
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HISP12-HRP | Alpha Diagnostics | 100 ul | EUR 347 |